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human primary brain vascular pericyte cells  (ScienCell)

 
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    ScienCell human primary brain vascular pericyte cells
    Human primary VSMC populations were screened for <t>pericyte</t> markers. A , Normal human aortic VSMC populations (n=4) number 70008916 (lane A) and number 70045161 (lane B) from American Type Culture Collection, #10 and #53 primary VSMC isolated from aortic biopsy adjacent to abdominal aneurysm, dVSMC (n=6) isolated from abdominal aortic aneurysm biopsy, and HBVPs, n=1 were cultured in 6‐well plates in growth medium until 70% to 80% confluency. Cells were lysed and cell lysates were subjected for immunoblotting analysis as indicated target proteins and followed by appropriate housekeeping protein HSP90 or tubulin. Target proteins were normalized to the housekeeping protein on the same membrane and graphed in ( B ). C through F , Primary human VSMC (American Type Culture Collection; no. 70008916), at passages 6 to 9 were subjected to adipogenic/lipogenic induction medium (smooth‐muscle cell growth medium 2 containing 250 μmol/L IBMX, 2 nmol/L T3, 100 nmol/L dexamethasone, 10 nmol/L hydrocortisone, 5 μmol/L indomethacin, 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone (Adipo‐R) or 5 μmol/L T0901317 (Lipo‐T) for 6 days, and then maintained in smooth‐muscle cell growth medium 2 containing 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone or 5 μmol/L T0901317, respectively, for another 6 days. Cells were fixed for oil red O staining, data representing 1 biological replicate, with methodologies performed in technical triplicate ( C ) and quantification of lipid ( D ). Cell lysates were subjected to immunoblotting to detect proteins indicated ( E ), and changes in relative protein levels normalized by appropriate housekeeping protein on the same blot quantified ( F ). G through K , The same experiment was performed with primary dVSMC isolated from abdominal aortic aneurysm (ID no. 64, biological [n=1]). G , Oil red O staining and quantification ( H ) after differentiation. I , Immunoblot with proteins indicated, with quantification in ( J ). K , Total RNA was collected for reverse transcriptase quantitative polymerase chain reaction as indicated. Relative mRNA levels were calculated using δδCt method (details in Methods section). Scale bars=200 μm. Statistical analysis was performed using a Brown‐Forsythe test prior to using 1‐way ANOVA followed by Tukey's post hoc multiple comparisons test. When evaluating sterol regulatory element‐binding protein‐1 protein abundance, a 2‐way ANOVA followed by Sidak's post hoc multiple comparisons test was conducted. Data are presented as mean±SD, with error bars representing the SD, analyses conducted in Prism 7 (GraphPad Software). Statistical significance was determined at * P <0.05; ** P <0.01; *** P <0.001. Ct indicates number of amplification cycles reaching the threshold fluorescence; dVSMC, <t>dilated</t> <t>vascular</t> smooth‐muscle cell; HBVP, primary human brain pericyte; IBMX, 3‐isobutyl‐1‐methylxanthine; and VSMC, vascular smooth‐muscle cell.
    Human Primary Brain Vascular Pericyte Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+primary+brain+vascular+pericyte+cells/human+primary+brain+vascular+pericyte+cells/pmc12132619-84-0-6
    Average 90 stars, based on 1 article reviews
    human primary brain vascular pericyte cells - by Bioz Stars, 2026-10
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    1) Product Images from "Exploration of Conserved Human Adipose Subpopulations Using Targeted Single‐Nuclei RNA Sequencing Data Sets"

    Article Title: Exploration of Conserved Human Adipose Subpopulations Using Targeted Single‐Nuclei RNA Sequencing Data Sets

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.124.038465

    Human primary VSMC populations were screened for pericyte markers. A , Normal human aortic VSMC populations (n=4) number 70008916 (lane A) and number 70045161 (lane B) from American Type Culture Collection, #10 and #53 primary VSMC isolated from aortic biopsy adjacent to abdominal aneurysm, dVSMC (n=6) isolated from abdominal aortic aneurysm biopsy, and HBVPs, n=1 were cultured in 6‐well plates in growth medium until 70% to 80% confluency. Cells were lysed and cell lysates were subjected for immunoblotting analysis as indicated target proteins and followed by appropriate housekeeping protein HSP90 or tubulin. Target proteins were normalized to the housekeeping protein on the same membrane and graphed in ( B ). C through F , Primary human VSMC (American Type Culture Collection; no. 70008916), at passages 6 to 9 were subjected to adipogenic/lipogenic induction medium (smooth‐muscle cell growth medium 2 containing 250 μmol/L IBMX, 2 nmol/L T3, 100 nmol/L dexamethasone, 10 nmol/L hydrocortisone, 5 μmol/L indomethacin, 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone (Adipo‐R) or 5 μmol/L T0901317 (Lipo‐T) for 6 days, and then maintained in smooth‐muscle cell growth medium 2 containing 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone or 5 μmol/L T0901317, respectively, for another 6 days. Cells were fixed for oil red O staining, data representing 1 biological replicate, with methodologies performed in technical triplicate ( C ) and quantification of lipid ( D ). Cell lysates were subjected to immunoblotting to detect proteins indicated ( E ), and changes in relative protein levels normalized by appropriate housekeeping protein on the same blot quantified ( F ). G through K , The same experiment was performed with primary dVSMC isolated from abdominal aortic aneurysm (ID no. 64, biological [n=1]). G , Oil red O staining and quantification ( H ) after differentiation. I , Immunoblot with proteins indicated, with quantification in ( J ). K , Total RNA was collected for reverse transcriptase quantitative polymerase chain reaction as indicated. Relative mRNA levels were calculated using δδCt method (details in Methods section). Scale bars=200 μm. Statistical analysis was performed using a Brown‐Forsythe test prior to using 1‐way ANOVA followed by Tukey's post hoc multiple comparisons test. When evaluating sterol regulatory element‐binding protein‐1 protein abundance, a 2‐way ANOVA followed by Sidak's post hoc multiple comparisons test was conducted. Data are presented as mean±SD, with error bars representing the SD, analyses conducted in Prism 7 (GraphPad Software). Statistical significance was determined at * P <0.05; ** P <0.01; *** P <0.001. Ct indicates number of amplification cycles reaching the threshold fluorescence; dVSMC, dilated vascular smooth‐muscle cell; HBVP, primary human brain pericyte; IBMX, 3‐isobutyl‐1‐methylxanthine; and VSMC, vascular smooth‐muscle cell.
    Figure Legend Snippet: Human primary VSMC populations were screened for pericyte markers. A , Normal human aortic VSMC populations (n=4) number 70008916 (lane A) and number 70045161 (lane B) from American Type Culture Collection, #10 and #53 primary VSMC isolated from aortic biopsy adjacent to abdominal aneurysm, dVSMC (n=6) isolated from abdominal aortic aneurysm biopsy, and HBVPs, n=1 were cultured in 6‐well plates in growth medium until 70% to 80% confluency. Cells were lysed and cell lysates were subjected for immunoblotting analysis as indicated target proteins and followed by appropriate housekeeping protein HSP90 or tubulin. Target proteins were normalized to the housekeeping protein on the same membrane and graphed in ( B ). C through F , Primary human VSMC (American Type Culture Collection; no. 70008916), at passages 6 to 9 were subjected to adipogenic/lipogenic induction medium (smooth‐muscle cell growth medium 2 containing 250 μmol/L IBMX, 2 nmol/L T3, 100 nmol/L dexamethasone, 10 nmol/L hydrocortisone, 5 μmol/L indomethacin, 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone (Adipo‐R) or 5 μmol/L T0901317 (Lipo‐T) for 6 days, and then maintained in smooth‐muscle cell growth medium 2 containing 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone or 5 μmol/L T0901317, respectively, for another 6 days. Cells were fixed for oil red O staining, data representing 1 biological replicate, with methodologies performed in technical triplicate ( C ) and quantification of lipid ( D ). Cell lysates were subjected to immunoblotting to detect proteins indicated ( E ), and changes in relative protein levels normalized by appropriate housekeeping protein on the same blot quantified ( F ). G through K , The same experiment was performed with primary dVSMC isolated from abdominal aortic aneurysm (ID no. 64, biological [n=1]). G , Oil red O staining and quantification ( H ) after differentiation. I , Immunoblot with proteins indicated, with quantification in ( J ). K , Total RNA was collected for reverse transcriptase quantitative polymerase chain reaction as indicated. Relative mRNA levels were calculated using δδCt method (details in Methods section). Scale bars=200 μm. Statistical analysis was performed using a Brown‐Forsythe test prior to using 1‐way ANOVA followed by Tukey's post hoc multiple comparisons test. When evaluating sterol regulatory element‐binding protein‐1 protein abundance, a 2‐way ANOVA followed by Sidak's post hoc multiple comparisons test was conducted. Data are presented as mean±SD, with error bars representing the SD, analyses conducted in Prism 7 (GraphPad Software). Statistical significance was determined at * P <0.05; ** P <0.01; *** P <0.001. Ct indicates number of amplification cycles reaching the threshold fluorescence; dVSMC, dilated vascular smooth‐muscle cell; HBVP, primary human brain pericyte; IBMX, 3‐isobutyl‐1‐methylxanthine; and VSMC, vascular smooth‐muscle cell.

    Techniques Used: Isolation, Cell Culture, Western Blot, Membrane, Staining, Reverse Transcription, Real-time Polymerase Chain Reaction, Binding Assay, Quantitative Proteomics, Software, Amplification, Fluorescence

    Related Articles

    Cell Culture:

    Article Title: Exploration of Conserved Human Adipose Subpopulations Using Targeted Single‐Nuclei RNA Sequencing Data Sets
    Article Snippet: Immunofluorescence staining was performed using antibodies detailed in Table , including those detecting cluster of differentiation (CD) 31 (Cell Marque, Rocklin, CA), chondroitin sulfate proteoglycan 4 (NG2; Abcam, Waltham, MA), PDGFRB (Cell Signaling Technology, Danvers, MA [n=3]), RGS5 (Invitrogen, Waltham, MA [n=3]), transient receptor potential cation channel subfamily C member 6 (TRPC6; Proteintech, Chicago, IL [n=3]), ATP binding cassette subfamily C member 9 (ABCC9; LSBio, Newark, CA [n=3]), or COL25A1 (LSBio [n=4]) at 4°C overnight, followed by goat fluorescein isothiocyanate–anti‐mouse IgG (Vector Laboratories, Newark, CA) and Alexa fluor 647–anti‐rabbit IgG (Molecular Probes, Eugene, OR) or fluorescein isothiocyanate–anti‐rabbit IgG (Vector Laboratories) and donkey Rhodamine X‐anti‐mouse immunoglobulin G (Jackson ImmunoResearch Laboratories, West Grove, PA). .. Human primary brain vascular pericyte cells (ScienCell Research Laboratories, Carlsbad, CA; catalog no. 1200) were cultured in pericyte medium (basal medium, catalog no. 1201‐b; growth supplement, catalog no. 1252, and 2% FBS). .. Images were captured using a Leica SP8 confocal microscope.

    Article Title: Exploration of Conserved Human Adipose Subpopulations Using Targeted Single‐Nuclei RNA Sequencing Data Sets
    Article Snippet: Assay In Vitro Human VSMCs purchased from American Type Culture Collection (Manassas, VA; catalog no. PCS- 100- 012) and primary VSMCs isolated by explant outgrowth25 from dilated and nondilated aortic arteries from abdominal aortic aneurysm were cultured in smoothmuscle cell growth medium 2 (Lonza, Portsmouth, NH; catalog no. 3182) with SmGMTM- 2 Single Quots (Lonza; catalog no. 4149; and 5% FBS). .. Human primary brain vascular pericyte cells (ScienCell Research Laboratories, Carlsbad, CA; catalog no. 1200) were cultured in pericyte medium (basal medium, catalog no. 1201- b; growth supplement, catalog no. 1252, and 2% FBS). ..



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    Human primary VSMC populations were screened for <t>pericyte</t> markers. A , Normal human aortic VSMC populations (n=4) number 70008916 (lane A) and number 70045161 (lane B) from American Type Culture Collection, #10 and #53 primary VSMC isolated from aortic biopsy adjacent to abdominal aneurysm, dVSMC (n=6) isolated from abdominal aortic aneurysm biopsy, and HBVPs, n=1 were cultured in 6‐well plates in growth medium until 70% to 80% confluency. Cells were lysed and cell lysates were subjected for immunoblotting analysis as indicated target proteins and followed by appropriate housekeeping protein HSP90 or tubulin. Target proteins were normalized to the housekeeping protein on the same membrane and graphed in ( B ). C through F , Primary human VSMC (American Type Culture Collection; no. 70008916), at passages 6 to 9 were subjected to adipogenic/lipogenic induction medium (smooth‐muscle cell growth medium 2 containing 250 μmol/L IBMX, 2 nmol/L T3, 100 nmol/L dexamethasone, 10 nmol/L hydrocortisone, 5 μmol/L indomethacin, 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone (Adipo‐R) or 5 μmol/L T0901317 (Lipo‐T) for 6 days, and then maintained in smooth‐muscle cell growth medium 2 containing 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone or 5 μmol/L T0901317, respectively, for another 6 days. Cells were fixed for oil red O staining, data representing 1 biological replicate, with methodologies performed in technical triplicate ( C ) and quantification of lipid ( D ). Cell lysates were subjected to immunoblotting to detect proteins indicated ( E ), and changes in relative protein levels normalized by appropriate housekeeping protein on the same blot quantified ( F ). G through K , The same experiment was performed with primary dVSMC isolated from abdominal aortic aneurysm (ID no. 64, biological [n=1]). G , Oil red O staining and quantification ( H ) after differentiation. I , Immunoblot with proteins indicated, with quantification in ( J ). K , Total RNA was collected for reverse transcriptase quantitative polymerase chain reaction as indicated. Relative mRNA levels were calculated using δδCt method (details in Methods section). Scale bars=200 μm. Statistical analysis was performed using a Brown‐Forsythe test prior to using 1‐way ANOVA followed by Tukey's post hoc multiple comparisons test. When evaluating sterol regulatory element‐binding protein‐1 protein abundance, a 2‐way ANOVA followed by Sidak's post hoc multiple comparisons test was conducted. Data are presented as mean±SD, with error bars representing the SD, analyses conducted in Prism 7 (GraphPad Software). Statistical significance was determined at * P <0.05; ** P <0.01; *** P <0.001. Ct indicates number of amplification cycles reaching the threshold fluorescence; dVSMC, <t>dilated</t> <t>vascular</t> smooth‐muscle cell; HBVP, primary human brain pericyte; IBMX, 3‐isobutyl‐1‐methylxanthine; and VSMC, vascular smooth‐muscle cell.
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    Human primary VSMC populations were screened for pericyte markers. A , Normal human aortic VSMC populations (n=4) number 70008916 (lane A) and number 70045161 (lane B) from American Type Culture Collection, #10 and #53 primary VSMC isolated from aortic biopsy adjacent to abdominal aneurysm, dVSMC (n=6) isolated from abdominal aortic aneurysm biopsy, and HBVPs, n=1 were cultured in 6‐well plates in growth medium until 70% to 80% confluency. Cells were lysed and cell lysates were subjected for immunoblotting analysis as indicated target proteins and followed by appropriate housekeeping protein HSP90 or tubulin. Target proteins were normalized to the housekeeping protein on the same membrane and graphed in ( B ). C through F , Primary human VSMC (American Type Culture Collection; no. 70008916), at passages 6 to 9 were subjected to adipogenic/lipogenic induction medium (smooth‐muscle cell growth medium 2 containing 250 μmol/L IBMX, 2 nmol/L T3, 100 nmol/L dexamethasone, 10 nmol/L hydrocortisone, 5 μmol/L indomethacin, 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone (Adipo‐R) or 5 μmol/L T0901317 (Lipo‐T) for 6 days, and then maintained in smooth‐muscle cell growth medium 2 containing 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone or 5 μmol/L T0901317, respectively, for another 6 days. Cells were fixed for oil red O staining, data representing 1 biological replicate, with methodologies performed in technical triplicate ( C ) and quantification of lipid ( D ). Cell lysates were subjected to immunoblotting to detect proteins indicated ( E ), and changes in relative protein levels normalized by appropriate housekeeping protein on the same blot quantified ( F ). G through K , The same experiment was performed with primary dVSMC isolated from abdominal aortic aneurysm (ID no. 64, biological [n=1]). G , Oil red O staining and quantification ( H ) after differentiation. I , Immunoblot with proteins indicated, with quantification in ( J ). K , Total RNA was collected for reverse transcriptase quantitative polymerase chain reaction as indicated. Relative mRNA levels were calculated using δδCt method (details in Methods section). Scale bars=200 μm. Statistical analysis was performed using a Brown‐Forsythe test prior to using 1‐way ANOVA followed by Tukey's post hoc multiple comparisons test. When evaluating sterol regulatory element‐binding protein‐1 protein abundance, a 2‐way ANOVA followed by Sidak's post hoc multiple comparisons test was conducted. Data are presented as mean±SD, with error bars representing the SD, analyses conducted in Prism 7 (GraphPad Software). Statistical significance was determined at * P <0.05; ** P <0.01; *** P <0.001. Ct indicates number of amplification cycles reaching the threshold fluorescence; dVSMC, dilated vascular smooth‐muscle cell; HBVP, primary human brain pericyte; IBMX, 3‐isobutyl‐1‐methylxanthine; and VSMC, vascular smooth‐muscle cell.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Exploration of Conserved Human Adipose Subpopulations Using Targeted Single‐Nuclei RNA Sequencing Data Sets

    doi: 10.1161/JAHA.124.038465

    Figure Lengend Snippet: Human primary VSMC populations were screened for pericyte markers. A , Normal human aortic VSMC populations (n=4) number 70008916 (lane A) and number 70045161 (lane B) from American Type Culture Collection, #10 and #53 primary VSMC isolated from aortic biopsy adjacent to abdominal aneurysm, dVSMC (n=6) isolated from abdominal aortic aneurysm biopsy, and HBVPs, n=1 were cultured in 6‐well plates in growth medium until 70% to 80% confluency. Cells were lysed and cell lysates were subjected for immunoblotting analysis as indicated target proteins and followed by appropriate housekeeping protein HSP90 or tubulin. Target proteins were normalized to the housekeeping protein on the same membrane and graphed in ( B ). C through F , Primary human VSMC (American Type Culture Collection; no. 70008916), at passages 6 to 9 were subjected to adipogenic/lipogenic induction medium (smooth‐muscle cell growth medium 2 containing 250 μmol/L IBMX, 2 nmol/L T3, 100 nmol/L dexamethasone, 10 nmol/L hydrocortisone, 5 μmol/L indomethacin, 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone (Adipo‐R) or 5 μmol/L T0901317 (Lipo‐T) for 6 days, and then maintained in smooth‐muscle cell growth medium 2 containing 1.25 μmol/L insulin, 33 μmol/L biotin with either 1 μmol/L rosiglitazone or 5 μmol/L T0901317, respectively, for another 6 days. Cells were fixed for oil red O staining, data representing 1 biological replicate, with methodologies performed in technical triplicate ( C ) and quantification of lipid ( D ). Cell lysates were subjected to immunoblotting to detect proteins indicated ( E ), and changes in relative protein levels normalized by appropriate housekeeping protein on the same blot quantified ( F ). G through K , The same experiment was performed with primary dVSMC isolated from abdominal aortic aneurysm (ID no. 64, biological [n=1]). G , Oil red O staining and quantification ( H ) after differentiation. I , Immunoblot with proteins indicated, with quantification in ( J ). K , Total RNA was collected for reverse transcriptase quantitative polymerase chain reaction as indicated. Relative mRNA levels were calculated using δδCt method (details in Methods section). Scale bars=200 μm. Statistical analysis was performed using a Brown‐Forsythe test prior to using 1‐way ANOVA followed by Tukey's post hoc multiple comparisons test. When evaluating sterol regulatory element‐binding protein‐1 protein abundance, a 2‐way ANOVA followed by Sidak's post hoc multiple comparisons test was conducted. Data are presented as mean±SD, with error bars representing the SD, analyses conducted in Prism 7 (GraphPad Software). Statistical significance was determined at * P <0.05; ** P <0.01; *** P <0.001. Ct indicates number of amplification cycles reaching the threshold fluorescence; dVSMC, dilated vascular smooth‐muscle cell; HBVP, primary human brain pericyte; IBMX, 3‐isobutyl‐1‐methylxanthine; and VSMC, vascular smooth‐muscle cell.

    Article Snippet: Human primary brain vascular pericyte cells (ScienCell Research Laboratories, Carlsbad, CA; catalog no. 1200) were cultured in pericyte medium (basal medium, catalog no. 1201‐b; growth supplement, catalog no. 1252, and 2% FBS).

    Techniques: Isolation, Cell Culture, Western Blot, Membrane, Staining, Reverse Transcription, Real-time Polymerase Chain Reaction, Binding Assay, Quantitative Proteomics, Software, Amplification, Fluorescence